1. Plant

• species, cultivar and stage of development

• day/night pattern

• photosynthetic capability

• stress sensitivity

3.Culture-container

• characteristics of body and closure device

• material, structure, thickness, shape, etc.

• permeability for gases and water vapour

• mechanisms of gas transfer

• headspace volume and plant density

2. Medium formulation

• state: liquid or solidified

• partition and diffusion coefficients (solubility)

• external carbon source, hormonal balance, nutrient supply

• use of inhibitors of biosynthesis and action, air scrubbers

4. Environmental conditions

• temperature, pressure, light, relative humidity and gaseous composition

  • in- and outside the container (spatial variation)

  • day- and night cycles

  • influence of bottom cooling, ventilation, etc.

• Contamination