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Influence of species and stage of development
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Under comparable conditions, the headspace composition is species and cultivar specific. Also the stage of development (multiplication or rooting) results in another headspace evolution and composition. Both factors are illustrated in the figures below in which the evolution of CO2 and ethylene is followed. |

Fig 1. Evolution of CO2 in the headspace of the tissue culture vessel during the multiplication stage for several plant species

Fig 2. Evolution of CO2 in the headspace of the tissue culture vessel during the rooting stage for several plant species

Fig 3. Evolution of ethylene in the headspace of the tissue culture vessel during the multiplication stage for several plant species

Fig 4. Evolution of ethylene in the headspace of the tissue culture vessel during the rooting stage for several plant species