Headspace composition
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Headspace composition |
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The headspace compostion in a tissue culture container can be significantly different from the greenhouse atmosphere, as shown in the table. Other volatiles are present in smaller amounts. |
|
component |
greenhouse environment |
container atmosphere |
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O2 |
22 % |
as low as 4 % |
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N2 |
77 % |
up to 87 % |
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CO2 |
365 - 1000 ppm |
up to 20 % |
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water vapour |
60-85 % |
± 100 % |
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ethylene |
5 ppb - 100 ppb |
> 2 ppm |
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The headspace composition influences plant growth and development: e.g.
Factors determining the gaseous compositon of an in vitro culture container: |