Turning points in research work 研究工作的转折点

You do need to have the ability to spot turning points in any project of your endeavour. In my case I spotted the turning point to make little bulbs out of my daffodils/narcissus in test tubes. And my PhD supervisors made sure I scaled the most important turning point – the completion of my PhD thesis on schedule!
你确实需要有能力在你努力的任何科研项目中发现转折点。在我的例子中,我发现的一个转折点就是怎样使得试管培养的水仙花芽丛形成小鳞茎。我的两位博士导师确保我完成了最重要的转折点:就是如期完成博士论文!

About 35 years ago, having cracked how I could make a lot of daffodil shoots in test tubes (that’s another story!), I was faced with a great hurdle that for the life of me I had no idea how to tackle. How could I turn these shoot clumps into little bulbs for planting in the field? After all, my PhD’s goal was to produce a complete protocol from multiplying massive number of daffodils/narcissus shoot clumps in test-tubes and getting these growing ‘normally’ in the field. Based on literature reviews, I knew that plating shoot clumps had been successfully done by other researchers. But this had two major disadvantages:

    • There would be a danger of these shoot clumps not acclimatizing well when planted out in the glass house. 25 – 30% casualties would be “normal”.
    • These shoot clumps were behaving like young narcissus seedlings and would need up to five years to grow to flowering stage.

My late supervisor, Dr. Barbara M.R. Harvey suggested I should look at other plant models in tissue culture for inspirations. The light-bulb moment came when I had a chat with Dr. Nikki Evans on how she got her potato shoots in test-tubes to form small tubers. Evans used high sugar content in her culture medium and I went along this line. A few months later, I was elated to see little bulbs (which I termed ‘bulbils”) formed in my test-tubes given high dose of sucrose in their culture media. That was the turning point of my PhD and 6 months later I submitted my thesis…and the rest as they say “was history”!

Narcisus in various stages of bulb formation
Narcisus shoots in various stages of forming bulbils. From left: Starting with single leaf with basal plate tissue; after 3 – 4 weeks of culture duration signs of bulbil formation were clear; after 6 – 7 weeks a bulbil (about 1 cm in diameter) was formed; after 10 weeks of culture the bulbil had grown to be around 2 cm in diameter, the equivalent size of a one-year-old seedling. [Photo’s copyright: Dr. Chow Yong Neng]
For this part of my work I am indebted to Dr. Evans for her generosity in sharing her ideas.

I owed it to my two supervisors, Dr. Barbara Harvey and Dr. Christopher Selby for putting their feet down shortly after I had completed the “bulbils” experiment by stopping me from doing any more work in the lab (they literally banished me from my lab!) and by pushing me to write my doctorate thesis which I completed about 6 months later! This was the most important turning point! My research project goals were attained, but my doctoral studies goal was still not reached. I had to write, submit and  defend my thesis (successfully) to earn my PhD!

A few months after that (in December 1990), I was conferred the degree of Doctor of Philosophy by my alma mater, the Queen’s University of Belfast. Shortly after, I left Belfast to take up a post-doctoral research officer post at the National University of Singapore.

It was around late 1991 that I received the great news from Dr. Harvey, she wrote in an email: “Chow, some of the “bulbils” that you had planted out in mid 1990 are flowering!”

This was exciting news indeed because:

    • it proofed that my bulbils, after going through the “stressful” process of my protocol were not any different from those multiplied conventionally;
    • of more significance is the fact that this meant my protocol had cut the “shoot clumps/seedlings to flowering bulb stage”by at least 3 – 4 years!

I wished I had the chance and resources to carry on with this work to collect more data on this observation but disappointingly this was not the case.

In research work, you do need to have a keen eye to spot trends & changes but what you need most is the guidance of experienced researchers. I was lucky to have both!  The keen eye helped me to spot the resemblance between the potato tuberization process and the bulb formation of narcissus/daffodils. The guidance of my supervisors ensured that I scaled the last but most crucial turning point of my PhD journey. They put a stop to my laboratory work and made sure that I stuck to the PhD research schedule (and more importantly, my scholarship tenure) to compose and submit my PhD thesis way before the last of my scholarship cheques was issued!

If you are interested, a brief introduction to a paper published by my supervisors and I have just been written & published in Kudos by me.

中文版:

大约35年前,我成功的在试管里培养出了很多水仙花的嫩芽(其实这是首个转折点,也是另一个故事!),我面临着一个巨大的障碍,费尽了心思我根本不知道该如何去解决。我怎么能把这些芽丛变成小鳞茎以便他们可以顺利的在田间栽种呢?毕竟,我博士科研的目标是产生一个完整的方案,通过组织培养大量的生产水仙花芽丛,并使这他们可以在田间“正常”生长。根据文献回顾,我知道其他研究人员已经成功地将组织培养的水仙芽丛栽种到田间。但栽种这组培水仙芽丛有两个主要缺点:

● 将这些芽丛从试管移植到温室中,有它们不能很好地适应环境的危险。25-30%的损失将是“正常的”。

● 这些芽丛的本质就像刚发芽的水仙幼苗,将需要大概长达5年时间从成长到开花阶段。

我已故的导师芭芭拉·哈维博士建议我从其他的组织培养植物模型中寻找灵感。当我和尼基·埃文斯博士聊到她是如何在试管中培育出小块茎的时候,我灵光一闪。埃文斯博士在她组织培养土豆实验中使用了高含糖量的培养基就使得小土豆在试管内形成,我就跟随她的实验方向这样做。几个月后,我欣喜地看到了我实验试管里有些芽丛已经有所变化:可以观察到在我给予高剂量的蔗糖培养基的水仙芽丛里有小鳞茎(我称之为“珠芽”)的形成。那就是我读博士的另一个转折点。6个月后,我成功的提交了我的博士论文…其余的,就是英语术语说的”是历史”!

水仙芽丛在形成珠芽的不同阶段
水仙芽丛在形成珠芽的不同阶段。从左至右:从带有基板组织的单叶开始,培养3-4周后珠芽形成的迹象明显;6至7周后形成珠芽(直径约1 cm);培养10周后,珠芽已长到直径约2 cm,相等于一年生幼苗的大小。【照片版权:周永能博士】

在我的这一部分工作中,我要感谢埃文斯博士慷慨地分享她的想法。

我的两位博士导师,芭芭拉·哈维博士和克里斯托弗·塞尔比博士在我完成了“珠芽”实验后不久就很精明的阻止了我在实验室里做更多的实验(他们确实是把我“赶出“了实验室!) 。通过他们的催促,我全心全力的写好我的博士论文,大约6个月后我完成了这篇论文!这是最重要的转折点!因为当时我的研究项目目标已经实现,但我的博士研究目标仍未达到。为了获得博士学位,我必须写作、提交和成功的答辩我的论文!

几个月后(1990年12月),我被母校贝尔法斯特女王大学授予哲学博士学位。不久之后,我离开贝尔法斯特,到新加坡国立大学担任博士后研究员。

大约在1991年底,我收到了哈维博士的好消息,她在一封电子邮件中写道:“小周,你在1990年中所种下的一些‘珠芽’正在开花了!”

这确实是一个令人兴奋的消息,因为:

● 它证明了,在经历了我组织培养环境的“压力”及过程之后,我的珠芽和那些按惯例所被繁殖的鳞茎是没有什么不同;

● 更重要的是,这意味着我的方式方法成功的将“芽丛/幼苗到开花鳞茎期”缩短了至少3到4年!

当时我希望我有机会和资源在这方面进行更深入的研究工作,收集更多的数据,但令人失望的是,这已经是不可能的。

在研究工作中,你确实需要洞察力去敏锐地观察趋势和变化,但你最需要的是有经验研究人员的指导。我很幸运两者都有!敏锐的眼睛帮助我发现了土豆块茎化过程和水仙花鳞茎形成之间的相似之处。在导师的指导下,我攀登了博士之旅的最后一个但也是最关键的转折点。我的两位导师在我完成了“珠芽实验”后就停止了我的实验室工作,并确保我跟随学校设定博士研究时间表(更重要的是,我的奖学金任期),在我的最后一份奖学金支票被发出之前完成撰写和提交我的博士论文!

如果你有兴趣,我刚刚在Kudos杂志上发表了一篇文章是描述我两位导师和本人发表的一篇有关水仙芽丛形成珠芽的论文。

Plagiarism is stealing

Plagiarism is an act of presenting other people’s work or findings as one’s own. Plainly speaking, the perpetrator is a CHEAT. Nothing more and nothing less. In this article I will share with you my first hand experience as a victim of plagiarism inflicted with a double whammy of receiving a large dose of bullying and intimidation added in by the perpetrator to spice things up!

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Commentary (Mar 07, 2017):
I thought about writing something about my experience of being a victim of plagiarism plus bullying when I was working on my PhD. So in May 2016 I dug up all the facts and put together this article which first appeared in Han Chiang News where I served as the editor-in-chief cum Principal & CEO (well I needed to multi-roles to conserve cash for the college).

It was around late 1989 / early 1990. I was frantically getting as much of my laboratory work done, data taken and analysis completed as possible in order to beat the deadline set by the end of my scholarship funding. Then someone, whom I had held with great respect did the unimaginable: he plagiarized my work. When confronted by me, he further issued a personal threat to me. It was more hurting for the fact that this bully, plagiarist and cheat came from my supervisors’ group. Here it was, a man I looked up to when I was an undergraduate when he was one of my laboratory class demonstrators and a friend did this to me. I had literally helped this man get his job by giving him a quick tuition on the arts and science of plant tissue culture just a year before when he attended his job interview for a job opening that had my name to it (as it was in my area of expertise, but I did not qualify due to my status as a student). So I was pretty pleased when I learned that my so-called friend got this job. Little did I know that this “friend” would plagiarize my work later and put so much anxiety on me at the final leg of my PhD studies.

If I can arouse the courage and alertness of fellow victims of plagiarism to stand up like I did amidst personal threats and intimidation, then I have achieved my aim of writing this article. Hence I hope more plagiarists will have their acts of cheating exposed in the future if their victims take courage from what I did in 1990. With today’s modern anti-plagiarism tools such as Turnitin and powerful search engines like Google, it is getting easier to catch the cheats. I do hope more plagiarism scandals can be exposed!


University-endorsed plagiarism?

The recent case of a well known public university seemingly endorsing official plagiarism by its students hogged the headlines of the print media for a while.

What this story did was put forth the greatest of all academic sins; cheating.

Plagiarism is an act of presenting other people’s work or findings as one’s own. Plainly speaking, the perpetrator is a CHEAT. Nothing more and nothing less.

In the digital age of today, academic plagiarism can be committed easily. You just have to Google the topic of your quest and viola! You will get pages upon pages of links from Google to copy from.

I feel that we need to teach students right from primary school (even primary 1 or 2 is not too early a start) that simply copying things from the internet and presenting it as your own is wrong. Since this obviously has not been the case, we  now have university students and graduate students committing this act of cheating and not thinking twice about it.

The recent plagiarism case only came to light after one of the original owners/writers of the Master’s theses spotted his and his course mates’ work being branded as copyright of the said public university.

These victims’ work was submitted to another institution over a decade ago. Precisely since these original work was not “that old” (my thesis is 25 years old and it is still held in electronic format by the British Library), these work would have been indexed by anti-plagiarism services, the most famous of which is Turnitin.

One question begs an answer from this public university: did their staff put their students’ work through Turnitin (or similar services) before accepting them as the real McCoy? If not, why not?

In my humble opinion, this institution is just as guilty as their students or more so since they seemed to have condoned this act of plagiarism until the crime was exposed by one of the original authors.

Was it just an act of carelessness on the part of this public university’s official involved or is there a big loophole that students of this particular institution have been exploiting?

Narcissus as depicted by Van Gogh’s painting as the undergrowth. My PhD work was on how to clone massive numbers of the bulbs of this plant for it to be commercialized in Northern Ireland.

I feel that the power that be must seek a detailed explanation from the highest official of this public university. Failing which all the academic awards, accreditation and hence the reputation of this public university (and more importantly, its graduates all through the years) are in jeopardy.

How does a victim of academic plagiarism feel?

So how does a victim of an act of plagiarism feel? I will share with you my first hand experience.

Towards the final phase of my PhD studies at Queen’s University of Belfast (around April/May of 1990), my supervisors, fearing that I would not have enough time to write up my thesis, practically banished me from my laboratory, as far as doing more laboratory work was concerned. I was however allowed to visit my beloved narcissus cultures in test-tubes and to monitor the growth room’s facilities housing these periodically.

One day, I was watching the BBC documentary programme on science on TV and was surprised to see a familiar face being interviewed. SMT was working on commercialisation of  a variety of plants by tissue culture and the programme was about the work of my department.

What was shocking to me was this: 4 racks (each with about 20 test-tubes) of my beloved narcissus cultures were prominently shown behind SMT’s head along with a few other bottles of cultures that were SMT’s.

The worst was to come

SMT presented to the interviewer that all the cultures, especially pointing at mine, were his work. Hence the plagiarism was shown on the BBC! My work  was shown on the BBC but the credit was claimed by SMT!

I was furious to say the least. I confronted SMT at the next day. Since both of us worked in the laboratories of my two supervisors’, if SMT were to apologise and had bought me a cup of tea at the canteen, I would have let the case rest.

After all, my work got the be shown on the BBC.

However what came out from SMT when I confronted him in front of a couple of witnesses was even more shocking.

“Chow, you are just a bl***y student. You are a bl***y foreigner.

“I am a staff and I shall do as I please. If you push on with this, I shall get the Home Office (the British term for immigration office) on you”, said SMT

He further told me that as a student I had no right to my work and that my supervisors had given their consent for him to use my cultures for the BBC interview. I later checked with both of my supervisors who informed me that they collectively had no objection to SMT approaching me to borrow my test-tubes to appear on the BBC show. They did not however permit SMT to claim my work as his.

I knew that my supervisors would be in a dilemma if I filed my complaint with them as SMT was one of their subordinates.

Finding a saviour & protector

Luckily for me, I was taught in my Master’s studies in biotechnology by Professor Li Wan Po who had helped many foreign students especially when it came to their welfare.

When I approached Professor Li, his advice was simple: lie low, keep away from SMT, get the PhD thesis written as fast and as best as I could and get through my viva vorce first.

Since by early 1990 I was given the permission to remain in the UK for an indefinite period, in simple words, the right of abode in the UK, SMT’s threat of sending the Home Office on me did not pose any real danger to me.

But, messing up my PhD studies was a huge threat indeed.

“Prof, what happens if SMT tries to sabotage my PhD viva vorce or something like that?” I asked, fearing for the worse.

Professor Li told me the fact that I had reported the case to him was good enough and if I faced any further threats from SMT, he will take me to see the vice chancellor directly.

With that assurance, I buried my head in my writing and completed my PhD thesis and passed my viva vorce with minor corrections a few months later.

Getting a pound of flesh?

When I received a letter from the Queen’s University’s Academic Council advising me that I had been admitted for the Winter Graduation for 1990, I finally was able to breathe a sigh of relief, one which I was holding for over 6 months.

I printed seven extra copies of my thesis and distributed these to the researchers and professors in England, Scotland and Holland who had helped me to formulate my research plan and generously shared their knowledge with me.

Next I waived the moratorium on the circulation of my PhD thesis, thereby putting my work literally in the public domain. All these were done with one thing in mind; to prevent SMT from further claiming my work as his.

I then lodged a formal complaint against SMT to the Deputy Chief Scientific Officer of the Northern Ireland Department of Agriculture (DANI), Professor Marks who was the second highest official of DANI scientific services.

DANI made joint appointments to academic staff to the Faculty of Agriculture and Food Science with Queen’s University of Belfast and essentially I was working in DANI’s laboratories for my PhD.

Plagiarist’s lucky escape

But before I had the chance to push my case fully to get “my pound of flesh”, I had to leave Northern Ireland suddenly.

I managed to secure a post-doctoral position at the National University of Singapore. I needed to relocate to Singapore fast if I wanted that job!

SMT got away with his crime with a rap on the knuckles. He wrote me a letter of apology very reluctantly as demanded by me.

My story is one in which the victim had to endure months of anxiety and the perpetrator of plagiarism did not get the punishment that he fully deserved.

Perhaps SMT was lucky that 1990 was still in the pre-internet era and I did not have the means to expose his crime further.

In addition, out of respect to both my supervisors (who had to continue working with SMT), I did not pursue this further with Queen’s University. The case was viewed as “settled” at the Department of Agriculture, Northern Ireland level.

One of the reasons for my bringing the case up was the fact that SMT was prepared to bully me in full knowledge that I was the “de-facto” leader of all the postgraduate students in the faculty.

I had spent about eight years at the university by 1990 and thus was the most senior of all the postgraduate students.

My altruistic instinct was to protect my fellow students, especially overseas postgraduate students from falling prey to SMT.

This I was proud to learn later that I had attained!