Causes and evocators

Many causes have been mentioned. However, many of these factors will only act to induce or evoke hyperhydricity when other conditions in the culture system are not optimized

Following categories of factors can be considered:

• Explant: species, cultivar; length of time since last subculture; number of subcultures; way of cutting; placement on the medium

• Medium: concentration and type of gelling agent; presence of a liquid layer; the level and type of cytokinin; salt concentration

• Container: gas composition and gas concentration

• Environment: temperature; light intensity and light quality; RH; air movement