For some types of cultures totipotentiality is exploited, not for others. Indeed some cultures simply rely on the development of pre-existing structures (bud, tuber, seed, ...). Types of cultures which are a demonstration of totipotentiality are: de novo formation of buds, roots or embryos; differentiation of calli, isolated cells or protoplasts to yield organs and finally maybe complete plants.

Development of
pre-existing structures

De novo formation
based on totipotentiality

Axillary bud

Adventitious bud

Tuberization

Flowering in vitro

Ovary development

Rhizogenesis

Grafting in vitro

Bulbil development

Seed germination

Embryogenesis