Style grafting

Aim: overcoming incompatibility barriers

Example: style grafting in Lilium

To obtain interspecific lily crosses both pre-fertilization and post-fertilization barriers have to been overcome. One of the techniques that are used to overcome these problems is in vitro style grafting.

Figure 11. Grafted style method pollination (A) style of Asiatic hybrid 'Esther' compatibly pollinated with 'Connecticut King' pollen and attached to an ovary of the Oriental hybrid 'Star Gazer' ; (B) style of 'Star Gazer', compatibly pollinated with Oriental no. 783613 pollen and attached to an 'Esther' ovary. Figure 12. Detail of graft end from situation (A) in Fig. 11. Both style ends are slightly separated to show the bundle of pollen tubes transferring the stylar surfaces. Bar = 0.2 cm. (Van Tuyl et al. 1991)