Sowing of bulb flowers

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It is often difficult to sterilize bulbs for micropropagation purposes as they are heavily contaminated from being in soil. Seeds on the other hand are easy to sterilize. Sterilized seeds can then be aseptically sown on a water-agar medium or on a 1/10 Murashige and Skoog medium and left to germinate.
The sterile seedling can then be cut into pieces and used as explant material. By placing the explants on different hormone combinations either callus formation or multiple shooting can be obtained as seen above with Babiana explants.
Shoots produced via multiple shooting are then separated into individual plants to develop a bulblet before hardening off. Generaly, such bulblets acclimatize easily.
A hardened plantlet,
and the vibrant flower of Babiana rubrocyanea.