|
Plating density of protoplasts |
| Protoplasts have a maximum as well as a minimum plating density for growth. | |
| The optimum plating efficiency (tobacco protoplasts) 5 x 104 protoplasts/cm3. Protoplasts fail to divide when plated at one tenth of this concentration |
haemocytometer |
| The concentration of protoplasts can be determined by Fuchs-Rosenthal modified haemocytometer with a field depth of 0.2 mm. It is possible to adjust the concentration of protoplasts to the appropriate level by dilution or concentrating after centrifugation. |
