- Fluorescein diacetate:
accumulates only inside
the plasmalemma of viable
protoplasts, can be detected
with fluorescence/UV
microscopy
- Evans blue: Intact viable
protoplasts, exclude the
Evans blue stain.
Impermeability of the cell
to Evans blue indicates
a living cell.
- Cyclosis or protoplasmic
streaming can be a measure
of viability.
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These suspension culture cells show the principle
of the fluorescein diacetate. Viable cells convert
the stain from an optically inactive from to an
optically active form that glows apple green when
viewed under UV light
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Inverted UV microscope
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