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Isotonic plasmolyticum |
| The chief function of the cell wall is to exert wall pressure on the protoplast preventing excessive water uptake and bursting of the cell. Before the cell wall is removed, the cell must be bathed in an isotonic plasmolyticum (mannitol or sorbitol 13 %, these sugar alcohols are less readily metabolised by plant cells). It may be advantageous to test a range of mannitol concentrations varying from 8 -15% (w/v) | 
Plasmolyzed cell |