Mechanical isolation of protoplasts
|
Mechanical isolation of protoplasts |
| Cut
plasmolyzed tissue and subsequent deplasmolysis results in expansion and
release of the protoplasts from the cut ends of the cell.
In practice this technique is difficult and the yield of viable protoplasts is meager. One advantage, however, is that the deleterious effects of the wall-degrading enzymes on the metabolism of the protoplasts are eliminated. |
Mechanical method of protoplast isolation |