Sequence of protoplast development

Freshly isolated
protoplasts from
rice hypocotyls		 The first division
of a rice protoplast
four days after
isolation		 Protoplast derived
cell clusters of rice
in agarose		 Protoplast derived
cell clusters of
rice in agarose
blocks floating
in culture medium		 Protoplast derived
rice plantlet growing
in a test tube