Sterilization of culture media

Thermostabile ingredients are autoclaved; thermolabile ones are filter sterilized

• Autoclaving: adequate autoclaving requires that the medium is maintained at a choosen temperature and at the corresponding pressure for a given time; e.g. 50 kPa corresponds to 112°C, and 100 kPa to 120°C
• Filter sterilization: disposable filter membranes are first autoclaved; the filter pore size is not greater than 0.22µm; sterilized solutions are filtered a sterile container.