mother plants,
drip-irrigated

young shoots are cut off

leaves are discarded,
petioles remain on the shoot

rinse with 80% ethanol,
sterilize in 10 % commercial bleach (NaOCl)
for 15 minutes

rinse 3x
with sterile
water

cut off basal end and top

cut nodes

each node contains an
axillary meristem

bring the node in a test tube

ready