Kotte and Robbins
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Kotte and Robbins |
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Kotte |
It was only when a new approach was taken in 1922 that progress was made in culturing plant tissue aseptically. The credit for this new approach lies with two men who simultaneously and independently conceived the idea to use tissue that was already meristematic as an explant source. These men were a German KOTTE and an American ROBBINS. KOTTE worked with excised root tips such as pea and maize placing these in a variety of nutrients which contained the salts of Knop's solution, glucose and several nitrogen compounds such as asparagine, alanine and meat extract. KOTTE obtained growth of root tips for a periods of up to 2 weeks but he did not subculture. ROBBINS on the other hand maintained maize roots in vitro for longer periods by subculturing but with time the growth of the cultures decreased and the cultures were lost. ROBBINS used yeast extract in his cultures but his choice of maize was unfortunate. |
Robbins |
