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Northern blotting |
| Northern blotting is similar
to Southern blotting but RNA is used. The mRNA is extracted from a plant and resolved according to size using agarose electrophoresis. The mRNA, which is single stranded, is transferred to a membrane in a similar way as the DNA is. The northern blot is then probed with the denatured cloned gene or cDNA. This technique is useful in revealing whether the size of the cloned cDNA is full length. |
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