Co-integration
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Co-integration |
| The goc is inserted into a plasmid containing a segment of the T-DNA. The resulting plasmid called a shuttle vector is then transformed into E. coli. Transformants are selected on the basis of antibiotic resistance encoded by genes contained in the plasmid. The plasmid is then transferred from E. coli to Agrobacterium by a process called conjugation. Once inside Agrobacterium the shuttle vector integrates into the Ti plasmid by means of homologous recombination of the T-DNA on both plasmids. The Agrobacterium containing the recombinant Ti plasmid is selected and used to infect plant cells. Marker genes contained in the Ti plasmid coding for antibiotic resistance and other physiological traits are used to select transformed plants. |
The co-integration method |
