Anther after culture initiation.
Anther wall has burst open and
globular embryos and developing
microspores are visible

Microscopic cross section of
an anther 7 days after culture
initiation. Anther wall has burst
open and globular embryos and
developing microspores are visible.
The original exine from the
developing microspores/pollen
grains are stained red.

Globular embryos are visible
14 days after initiation

Microscopic cross section of
an anther 14 days after culture
initiation. Globular embryos
are visible 14 days after initiation

Advanced heart shaped
(cylindrical) and torpedo stage
embryos are visible in the anther

Microscopic cross section.
Advanced heart shaped
(cylindrical) and torpedo stage
embryos are visible. Vascular
tissue is visible in some of
the embryos

Tobacco anther with rooted
haploid plantlets