Callus: Genetic instability


    Callus cultures suffer from the problem
    of genetic instability.This may result in
    variations in phenotypes within a same
    culture.Variations may have an epigenetic
    or a genetic basis.

Epigenetic changes - selective gene expression -
any change in phenotype which does not result
from an alteration in DNA. These changes are
inheritable in the sense that there is no transmission
of the change to meiotic progeny, but they are
heritable and stable in the sense that they can be
passed from one cell generation to the next (e.g.
cytokinin habituation).
Genetic changes -chromosomal aberrations,
nuclear fragmentation, and endoreduplication
(polyploids). The frequency of these abnormalities
usually increases with the age of the culture,
and the cultural conditions may act in a selective
manner. Certain aneuploid or polypiold cells might
gain an advantage in division rate over the normal
cells and proliferate to a greater extent.

Differences in callus cultures
with callus that was subcultured
from the same source (explant)